Rink Amide Mbha Resin - Omizzur Ltd

Background：

Peptide is a biologically active substance related to various cell functions in organisms. Its molecular weight is between amino acids and proteins. Peptides are made up of many amino acids combined through peptide bonds in a certain order.

Peptides is often used in functional analysis, antibody research, especially drug development and other fields. The solid-phase synthesis of peptides is from the N- to the C-terminus.

Solid-phase Synthesis of Peptides:

The solid-phase peptide synthesis method has become an effective method for peptide synthesis due to its convenient and rapid speed. It has also brought a revolution in organic synthesis and has become an new subject: solid-phase organic synthesis. The invention also promote the automation of peptide synthesis. The world's real peptide synthesizer appeared in the early 1980s.

Resin

In solid-phase synthesis, amino acids are attached one by one from the C-to the N-terminus of the peptide chain. During this process, the amino group of the growing peptide chain condenses with the carboxyl group of the next amino acid until the synthesis is complete. In order to avoid side reactions, we usually protect the amino groups of amino acids that are about to be attached to the peptide chain.

There are currently two main protection strategies, the Boc strategy (the α-amino acid group of amino acids is protected by Boc) and the Fmoc strategy (the α-amino group of amino acids is protected by Fmoc). The C-terminus of the growing peptide chain is connected to the solid-phase resin through a linking group. we call it "Linker".

Currently widely used resins include PAM, Rink Amide Mbha Resin, Wang, 2-Cl-Trt, Rink-Amid, etc. They are generally polystyrene-divinylbenzene materials, and most of them have a cross-linking degree of 1%. At this degree of cross-linking, the resin has good swelling properties in DMF, DCM, and the reactant molecules can move freely inside the resin. The unit of substitution degree is mmol/g. It indicates how many mmol reaction sites there are in 1g of resin. Different resins determine the stability of Linker.

Amino is the most commonly used resin for the preparation of peptide amides. We usually use Rink amide mbha resin, and the amino group is protected by Fmoc. When loading the first amino acid, the Fmoc is first removed and then the normal coupling wash and deprotection cycle is entered. The linker of Rink amide-resin is stable and requires high concentration of TFA for cleavage, so it will also produce side effects similar to Wang-resin. If the peptide chain contains Trp residues, similar side effects should be noted.

For the Fmoc method, alkali must be used to remove Fmoc after each coupling step, so the Linker of the resin must be alkali-stable, such as 2-Cl-Trt resin. While PMA and MBHA are used in the Boc method, most of them currently use the Fmoc method.

Note: The black balls in the picture are PS balls. Each resin needs to be swollen with a suitable solvent before use. Commonly used ones include DCM and DMF.

Rink Amide Mbha Resin Data

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Storage: 2 - 8 °C

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