Article Introduction:

This article is a guidelines for reconstitute peptides , with the aim of helping peptide researchers and enthusiasts to use peptides more conveniently. The article is about 1500 words and reading takes 3-4 minutes.

*** Omizzur is a professional peptide synthesis company which product ship worldwide. 

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What is a Peptide?

Peptides are composed of amino acids arranged in a certain order. Different peptides have different numbers and sequences of amino acids. Peptide synthesis is usually done in peptide laboratories such as Omizzur peptide labs. The usual methods for synthesizing peptides include solid-phase peptide synthesis, liquid-phase peptide synthesis, and microwave technology. The purity of synthetic peptides is usually above 98%, 99% above.

What are the Popular Peptides in Recent Years?

As suppliers of peptides, we are familiar with popular peptides in recent years, such as Semaglutide, Tirzepatide, Retatrutide, Melanotan II etc. We will introduce their usage and storage methods in this article.

A Complete Reconstitute Peptide Guideline - Step by Step

Preparation work  (peptide in vial):

1. Bottled peptides: such as Semaglutide

2. Sterile or antibacterial water

3. Sterile syringe

4. Alcohol swab

Lets Start-up:

1. Disinfect all materials in contact, wash hands, and check the integrity of peptide bottles.

2. Prepare the solution according to the required peptide concentration: If the dose of bottled peptide is 5mg, and you want to achieve a concentration of 1mg/ml, you will need to extract 5ml of sterile aqueous solution. Similarly. If 2mg/ml is required, you will need to extract 2.5ml of aqueous solution.

3. Inject sterile aqueous solution into a peptide vial, gently rotate the vial to ensure thorough mixing and dissolution. Avoid violent shaking. Let it stand for 15-30 minutes.

4. After peptide dissolution, extract the solution with a syringe and expel any bubbles. Store the remaining solution according to the peptide guidelines. It is recommended to use it as soon as possible. Long term storage can reduce the stability of peptides.

Peptide Dissolution (Laboratory research of peptides: this section is for peptide researchers to read)

1. It is recommended to first use a small amount of peptide to test the more suitable dissolution method. Only when the peptide is completely dissolved can the solution be added and diluted to the final concentration. Dissolve first, then add buffer solution. Note: always add peptides to an appropriate solvent and stir, and do not use other methods.

2. The solubility of peptides depends on their sequence and modification. We recommend taking a small amount of peptides for solubility testing before dissolving all peptides.

3. You can calculate the length of the peptide first. Generally speaking, peptides with less than 6 amino acids can be soluble in water; For peptides with a chain length exceeding 6 amino acids, the dissolution principle mainly depends on their total charge and hydrophobicity.

If the length of the polypeptide chain exceeds 6 amino acids, you can calculate the total charge of the polypeptide according to the table below:

4.Based on the positive and negative charge values, the following are recommended solvents:

Be Careful:

1. It is recommended to store the solution with a concentration of 1-2mg/mL. Once the solution is prepared, please store it in separate packages at -20 ° C or -80 ° C to avoid product failure caused by repeated freeze-thaw cycles.

2. Peptides stored in the solution are susceptible to bacterial degradation. It is recommended to dissolve the peptides in sterile water or filter and sterilize the peptide solution before storage.

3. Peptides containing Cys, Met, or Trp are prone to oxidation and should be soluble in anaerobic water or solvents, and stored in an anaerobic environment.

Stability and Storage of Peptides

Peptides are provided in freeze-dried form and should be stored at -20 ° C. Water absorption can lead to a decrease in the stability of peptides, so it is important to ensure that titanium products are stored in a closed and dry environment and protected with desiccants.

Do not use a frost free freezer to store peptides, as changes in humidity and temperature may affect stability. Before opening and using, it is recommended to place the peptide in a room temperature environment for at least 60 minutes to raise its temperature to room temperature. Do not repeatedly freeze thaw. It is not recommended to store peptides in solution form for a long time as it will reduce stability.

The stability of peptides in solution form is much lower than that in freeze-drying form. When using peptides containing the following amino acids, it is particularly important to pay attention to this: cysteine, methionine, tryptophan, asparagine, glutamine, and terminal glutamic acid. Therefore, we suggest that the storage time of peptide solutions should be as short as possible. To avoid repeated freeze-thaw cycles, the peptide solution can be divided into multiple parts and stored at -20 ° C.

The unused portion after thawing in each serving should be discarded. Peptides stored in solution occasionally undergo bacterial degradation. We suggest using sterile solutions or filtering peptide solutions through a 0.2 micron pore size microfilter to remove potential bacterial contamination as much as possible.

Preservation of Freeze-dried Peptides

All products labeled "keep freeze-dried" must be stored under freezing conditions, preferably at -20 ℃. If stored below -80 ℃, most peptides can maintain their activity for several years.

When using frozen products, the bottle or test tube should be raised to room temperature in a drying oven containing fresh desiccant before opening the lid. For products stored at -20 ° C, this process takes one hour or longer, depending on the size of the packaging. Otherwise, when the bottle is opened, water vapor enters, causing peptide condensation and reducing its stability. Once opened, it should be quickly weighed and immediately sealed to prevent deliquescence, especially for hydrophilic peptides.

Preservation of Peptide Solutions

The stability of peptide solutions is much worse than that of dry powder. To achieve the best results, the following principles should be followed:

Repeated freeze-thaw cycles can damage the activity of peptides, so it is recommended to pack them in small packages for storage. Thaw as much as needed, and discard the remaining after use.

Dissolve in sterile buffer solution with pH 5-7 and store at -20 ° C.

Peptides containing Cys, Met, Typ, Glu, and Asp are prone to oxidation and therefore must be stored in an environment free of oxidants.

Due to the ability of bacteria to degrade peptides, it is necessary to filter and sterilize before storage

FAQs:

1. How to conduct quality inspection on synthesized peptides?

Answer: All synthesized peptides have been analyzed by HPLC and MS, and corresponding detection reports have been provided. All peptides are purified using reverse phase chromatography, and the molecular weight of the peptide is determined by mass spectrometry to determine the correctness of the product. MS detection results can also display most of the main impurities. If necessary, peptide net content testing can also be provided, such as amino acid analysis or elemental analysis.

These methods can confirm the amino acid composition of peptides and can serve as supplementary methods for peptide confirmation. All delivered peptides meet the purity requirements of the customer. Peptides that did not meet the purity requirements were discarded. We also support third-party laboratory testing.

2. What are the typical salt forms of peptides? How can salt be converted or desalinated?

Answer: Most peptides are isolated and purified under the TFA system, so peptides are mostly in the form of TFA salts. Secondly, drug peptides generally have acetate and hydrochloride forms, and a very small number of drug peptides may have some special salt forms. The salt conversion methods are mostly ion exchange and HPLC, while desalination can be achieved using G25 columns. The prices of acetate and hydrochloride are generally 20% -30% higher than those of TFA.

3. What form of peptide is shipped? What are the data reports provided?

Omizzur's peptides are shipped in freeze-dried powder form and can be packaged according to customer needs. Each peptide provides MS and HPLC reports

At present, transportation methods include air freight, FedEx, EMS, etc. Different countries adopt different transportation methods. Please contact our customer service before placing an order.