Peptide synthesis is a process of repeated addition of amino acids, and the solid phase synthesis is usually synthesized from the C terminal (carboxyl terminal) to the N terminal (amino terminal). In the past, peptide synthesis was stopped in solution, called liquid phase synthesis. Now the use of peptide synthesis technology is becoming more and more common, so take a look at what are the misunderstandings of peptide synthesis technology.

Since Merrifield developed the successful SPPS method for solid phase peptide synthesis in 1963, it has been modified and perfected from time to time to become a common technique in peptide and protein synthesis , Many common units can be stopped by off-the-shelf peptide synthesis programs. For beginners, understanding the principles of peptide synthesis is beneficial for understanding the process of peptide synthesis and solving the problems encountered in synthesis. In simple terms, the most fundamental programmed peptide synthesis process is nothing more than resin swelling ,de-maintenance, coupling, de-maintenance, recoupling ... Other special compositions are built on this foundation. For beginners in peptide synthesis, there are several misunderstandings to pay attention to.

A brief analysis of the three misunderstandings of peptide synthesis technology

Peptide synthesis mistake one: the longer the synthesis time, the better. After years of exploration and optimization of the efficiency of peptide synthesis, the combined effect rate of each step can reach more than 99% in a short time, and too long response time has limited progress in the positive response yield but greatly increases the probability of side reaction attacks, so too long response time is not good.

Peptide synthesis mistake two: universal peptide reagents. Most of the peptides can be cut from the resin with a combination of peptide peptides, and the amino acids with special side chain maintenance often need to adjust this combination, while the polypeptide sequences without side chain maintenance require a solution of 98%TFA and 2% water to complete the combination of sensitive peptide peptides.

Peptide synthesis mistake three: the side chain maintenance of amino acids are the same. Amino acids with vivid side chains require maintenance in the synthesis and there are different choices depending on the maintenance group of the side chain. Choosing the right maintenance base can effectively reduce the difficulty of synthesis.

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