At present, peptide antigen and recombinant protein antigen are often used for antibody preparation, which have advantages and disadvantages, and need to be selected by fusion test. Recombinant protein antigens usually contain several different antigenic determinants, some of which are sequence determinants and some are structural determinants. clonal antibodies obtained by immunizing animals with denatured antigens are compounds of the antigen for each epitopes, and in general use can be used to test for either natural constructs or denatured target proteins.

A side benefit of using denatured proteins as immunogens is that denatured proteins are often more antigenic and can stimulate animals to produce a strong immune response. The protein used as the antigen target is generally selected to use the Escherichia coli expression management system, because the management system has the least cost in time and money. In order to better improve the possibility of expression of the target protein and the ease of purification, we sometimes only express a small fragment of the target protein, such as a special domain.

Specific domains of proteins

If the purpose of antigen preparation is solely for wb test, the selection of small peptides generated as antigens is a rapid economic development, but there is a risk of weak antigenicity or no reflection of genicity due to inappropriate selection of peptides, because antibody preparation must be a long cycle time, so the use of polypeptide antigen to prepare antigens. Two or three different peptide segments are often selected to ensure the passage rate of the test.

The purity of the peptide antigen for immunity is stipulated to be more than 80%, and although a higher purity can theoretically obtain a non-specific and stronger antigen, the animal has to make a lot of non-specific antibodies during the whole process of operation, which covers the profit generated by the purity of the antigen.

There are two common antigen media KLH and BSA is about the synthesis of peptides and recombinant proteins as antigens of different relevant content, if you need to in-depth analysis of the specific situation, master a lot of related peptide synthesis content, you can visit the official website of www.omizzur.com.

What is the difference between synthetic peptides and recombinant proteins as antigenic bodies? Omizzur Biotech as a professional manufacturer of custom peptide synthesis. we also supply peptide raw materials, more details, please visit the official website of www.omizzur.com ,enjoy your consultation.